Detailed information of TA system TA028991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1639294..1640078
• Replicon: chromosome
• Accession: NC_020516
• Organism: Azoarcus sp. KH32C

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0Q349
• Locus tag: AZKH_RS07245
• Protein ID: WP_015435099.1
• Coordinates: 1639581..1640078 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: H0Q348
• Locus tag: AZKH_RS07240
• Protein ID: WP_015435098.1
• Coordinates: 1639294..1639584 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZKH_RS07230	1635021..1638494	-	3474	WP_015435096.1	type I restriction-modification system endonuclease	-
AZKH_RS07235	1638914..1639189	+	276	Protein_1424	VapC toxin family PIN domain ribonuclease	-
AZKH_RS07240	1639294..1639584	+	291	WP_015435098.1	DUF1778 domain-containing protein	Antitoxin
AZKH_RS07245	1639581..1640078	+	498	WP_015435099.1	GNAT family N-acetyltransferase	Toxin
AZKH_RS26765	1640118..1640633	-	516	Protein_1427	M48 family metallopeptidase	-
AZKH_RS07255	1640925..1641386	-	462	WP_015435101.1	(2Fe-2S)-binding protein	-
AZKH_RS07260	1641361..1643676	-	2316	WP_015435102.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
AZKH_RS07265	1643864..1644538	-	675	WP_015435103.1	carbonic anhydrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1629218..1640507	11289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18354.17 Da
• Isoelectric Point: 10.5040

>T28991 WP_015435099.1 NC_020516:1639581-1640078 [Azoarcus sp. KH32C]
MSLRGPEPLGTQHRRDGFDCGIPALNDWLVRHARQAQGSGSAKTFVVADDERVAGYFSLTVGQVDTLEAPDRIRKGMGQY
PVPVVILARLAVSRQDQGRGIGFGMLQDAIRRTLLIAEQAGIRAMLTHPIDENAAKFYTRFGFIASPLREQQLLLLLKDA
RRWVK

Nucleotide

• Length: 498 bp

>T28991 NC_020516:1639581-1640078 [Azoarcus sp. KH32C]
ATGAGCTTGCGCGGTCCGGAGCCGCTGGGGACGCAGCATCGCCGCGATGGATTCGATTGCGGAATACCGGCGCTGAACGA
CTGGCTGGTTCGCCACGCCCGCCAGGCGCAAGGCAGCGGCTCCGCCAAGACCTTCGTCGTTGCCGACGATGAGCGAGTCG
CAGGCTATTTCAGCCTGACCGTTGGCCAGGTTGACACGCTCGAAGCGCCCGACCGCATCCGCAAGGGGATGGGACAGTAC
CCGGTGCCGGTGGTGATCCTCGCCCGGCTGGCGGTGTCGCGACAGGATCAGGGGCGTGGTATCGGCTTTGGCATGTTGCA
GGACGCGATCCGGCGCACGTTGCTGATTGCCGAGCAGGCTGGCATCCGTGCGATGCTGACCCATCCGATCGACGAAAATG
CGGCGAAGTTCTACACCCGCTTCGGGTTCATTGCTTCGCCCTTGCGAGAGCAGCAATTGCTGCTGCTCCTCAAGGATGCT
CGACGCTGGGTGAAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10685.13 Da
• Isoelectric Point: 5.8349

>AT28991 WP_015435098.1 NC_020516:1639294-1639584 [Azoarcus sp. KH32C]
MAAATSSAPRSARLGLRATPEQEAVLRRAAEVAHKSLTDFILDSACQAAEQTLLDQRLFMVTGSQYQALMDLLERPEQPN
EGLRDLFSRKAPWDVR

Nucleotide

• Length: 291 bp

>AT28991 NC_020516:1639294-1639584 [Azoarcus sp. KH32C]
ATGGCTGCTGCCACGTCATCAGCTCCCCGCTCCGCCCGACTGGGACTGCGCGCCACGCCCGAGCAAGAAGCCGTGTTGCG
GCGTGCCGCCGAGGTGGCGCACAAATCCTTGACCGATTTCATTCTCGACAGCGCATGCCAGGCTGCCGAGCAGACCCTGC
TCGATCAGCGCCTGTTCATGGTCACGGGTAGCCAATATCAAGCCTTGATGGATCTGCTGGAGCGGCCTGAGCAGCCCAAC
GAGGGCTTGCGCGATCTGTTCTCGCGCAAGGCTCCGTGGGATGTGAGATGA

Structures

Toxin

• AlphaFold DB: H0Q349

Antitoxin

• AlphaFold DB: H0Q348