Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-StbC
Location 1628060..1628700 Replicon chromosome
Accession NC_020516
Organism Azoarcus sp. KH32C

Toxin (Protein)


Gene name vapC Uniprot ID H0Q339
Locus tag AZKH_RS07190 Protein ID WP_015435089.1
Coordinates 1628272..1628700 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID H0Q338
Locus tag AZKH_RS07185 Protein ID WP_015435088.1
Coordinates 1628060..1628272 (+) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZKH_RS07155 1623269..1623877 + 609 WP_015435083.1 indolepyruvate oxidoreductase subunit beta -
AZKH_RS07160 1624146..1625018 + 873 WP_015435084.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
AZKH_RS07165 1625023..1625526 + 504 WP_015435085.1 5-(carboxyamino)imidazole ribonucleotide mutase -
AZKH_RS07170 1625529..1626680 + 1152 WP_041657047.1 5-(carboxyamino)imidazole ribonucleotide synthase -
AZKH_RS07175 1626667..1627740 + 1074 WP_015435087.1 threonylcarbamoyl-AMP synthase -
AZKH_RS07185 1628060..1628272 + 213 WP_015435088.1 hypothetical protein Antitoxin
AZKH_RS07190 1628272..1628700 + 429 WP_015435089.1 type II toxin-antitoxin system VapC family toxin Toxin
AZKH_RS26760 1630363..1630701 + 339 WP_083903157.1 thermonuclease family protein -
AZKH_RS07215 1630867..1632258 - 1392 WP_015435093.1 restriction endonuclease subunit S -
AZKH_RS07220 1632255..1633697 - 1443 WP_015435094.1 type I restriction-modification system subunit M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-125)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15729.81 Da        Isoelectric Point: 4.8836

>T28990 WP_015435089.1 NC_020516:1628272-1628700 [Azoarcus sp. KH32C]
MYLLDTNIISELRKGGRANPGVTAFFATLAAEDIYLSVQTIGEIRRGLDNIRGRGDRQQAERLEAWLDNVVADYSDRILG
FDLDCAQVWGKLMSPSAQHPIDKQIAAIALIYDLTVVTRNTEDFKTTGVRSINPFNSPLSTA

Download         Length: 429 bp

>T28990 NC_020516:1628272-1628700 [Azoarcus sp. KH32C]
ATGTACCTGCTCGACACCAATATCATCAGCGAGCTGCGCAAAGGCGGGCGCGCCAATCCAGGCGTTACGGCGTTCTTCGC
GACATTGGCTGCCGAGGACATTTATTTGTCGGTGCAGACCATCGGCGAGATTCGTCGAGGACTCGATAACATTCGCGGCC
GCGGGGACCGGCAGCAGGCCGAGCGGCTCGAGGCCTGGCTTGATAATGTGGTCGCCGACTATTCGGACCGGATTCTTGGC
TTCGACCTCGACTGCGCTCAGGTTTGGGGAAAGCTCATGTCACCGAGTGCCCAGCATCCCATCGACAAGCAGATTGCGGC
CATCGCTCTGATCTACGACCTGACGGTCGTCACGCGTAACACGGAAGATTTCAAGACGACGGGAGTGCGCAGCATCAATC
CCTTCAACTCGCCCTTATCGACGGCCTGA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7730.73 Da        Isoelectric Point: 9.0188

>AT28990 WP_015435088.1 NC_020516:1628060-1628272 [Azoarcus sp. KH32C]
MATNLIVRNVEPSVTQALKEAAARHGRSAEAEHREILRAALARPARRTFKDVLAGMPDVGTDEDFNFRQG

Download         Length: 213 bp

>AT28990 NC_020516:1628060-1628272 [Azoarcus sp. KH32C]
ATGGCGACCAATCTTATTGTCCGCAACGTCGAACCGTCGGTAACACAGGCCTTGAAGGAGGCCGCGGCACGGCACGGGCG
TAGCGCTGAAGCAGAGCACCGCGAAATCTTGCGCGCAGCCCTCGCGCGACCGGCGCGGCGAACCTTCAAGGACGTGCTGG
CCGGCATGCCGGATGTCGGTACCGATGAAGACTTCAACTTCAGGCAGGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0Q339

Antitoxin

Source ID Structure
AlphaFold DB H0Q338

References