Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3104832..3105402 Replicon chromosome
Accession NZ_LR882499
Organism Mycobacterium tuberculosis variant microti OV254 isolate vole

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN986_RS14540 Protein ID WP_003414166.1
Coordinates 3104832..3105188 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN986_RS14545 Protein ID WP_003901465.1
Coordinates 3105172..3105402 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN986_RS14520 3100284..3101972 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN986_RS14525 3101976..3102302 - 327 WP_003414157.1 hypothetical protein -
JN986_RS14530 3102475..3103062 + 588 WP_003914429.1 DUF3558 family protein -
JN986_RS14535 3103081..3104730 + 1650 WP_105799646.1 CocE/NonD family hydrolase -
JN986_RS14540 3104832..3105188 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN986_RS14545 3105172..3105402 - 231 WP_003901465.1 antitoxin MazE Antitoxin
JN986_RS14550 3105445..3106488 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
JN986_RS14555 3106679..3106954 + 276 WP_003911993.1 DUF1778 domain-containing protein -
JN986_RS14560 3107130..3107384 - 255 WP_003917684.1 hypothetical protein -
JN986_RS14565 3107532..3107936 + 405 WP_003414181.1 hypothetical protein -
JN986_RS14570 3107933..3108124 + 192 WP_003414184.1 hypothetical protein -
JN986_RS14575 3108188..3109477 + 1290 Protein_2876 transposase family protein -
JN986_RS14580 3109711..3109968 + 258 WP_003899489.1 hypothetical protein -
JN986_RS14585 3110073..3110384 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T289890 WP_003414166.1 NZ_LR882499:c3105188-3104832 [Mycobacterium tuberculosis variant microti OV254]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT289890 WP_003901465.1 NZ_LR882499:c3105402-3105172 [Mycobacterium tuberculosis variant microti OV254]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References