Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 491751..492388 Replicon
Accession NC_020410
Organism Bacillus amyloliquefaciens subsp. plantarum UCMB5036

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BAM5036_RS02460 Protein ID WP_003156187.1
Coordinates 492038..492388 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BAM5036_RS02455 Protein ID WP_003156188.1
Coordinates 491751..492032 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAM5036_RS02435 488116..488715 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
BAM5036_RS02440 488808..489173 + 366 WP_015416872.1 holo-ACP synthase -
BAM5036_RS02445 489338..490345 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BAM5036_RS02450 490462..491631 + 1170 WP_015416873.1 alanine racemase -
BAM5036_RS02455 491751..492032 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BAM5036_RS02460 492038..492388 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BAM5036_RS02465 492506..493327 + 822 WP_015416874.1 STAS domain-containing protein -
BAM5036_RS02470 493332..493697 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BAM5036_RS02475 493700..494101 + 402 WP_003156178.1 anti-sigma regulatory factor -
BAM5036_RS02480 494113..495120 + 1008 WP_015416875.1 PP2C family protein-serine/threonine phosphatase -
BAM5036_RS02485 495184..495513 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BAM5036_RS02490 495510..495992 + 483 WP_003156173.1 anti-sigma B factor RsbW -
BAM5036_RS02495 495958..496746 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BAM5036_RS02500 496746..497348 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T28978 WP_003156187.1 NC_020410:492038-492388 [Bacillus velezensis UCMB5036]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T28978 NC_020410:492038-492388 [Bacillus velezensis UCMB5036]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT28978 WP_003156188.1 NC_020410:491751-492032 [Bacillus velezensis UCMB5036]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT28978 NC_020410:491751-492032 [Bacillus velezensis UCMB5036]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References