Detailed information of TA system TA028969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-ChpS
• Location: 71288..71877
• Replicon: chromosome
• Accession: NC_020300
• Organism: Bartonella australis Aust/NH1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M1NRJ6
• Locus tag: BANH1_RS00315
• Protein ID: WP_015397462.1
• Coordinates: 71288..71620 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M1N227
• Locus tag: BANH1_RS00320
• Protein ID: WP_015397463.1
• Coordinates: 71620..71877 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BANH1_RS00270	66929..67387	+	459	WP_015397454.1	conjugal transfer protein TraM	-
BANH1_RS00275	67460..67783	-	324	WP_015397455.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BANH1_RS00280	67783..68007	-	225	WP_019224016.1	antitoxin MazE family protein	-
BANH1_RS00285	68151..68402	+	252	WP_015397457.1	hypothetical protein	-
BANH1_RS00290	68399..68797	+	399	WP_015397458.1	type II toxin-antitoxin system VapC family toxin	-
BANH1_RS00295	69084..69602	+	519	WP_015397459.1	replication/maintenance protein RepL	-
BANH1_RS00300	69762..70019	-	258	WP_015397460.1	hypothetical protein	-
BANH1_RS00305	70016..70666	-	651	WP_015397461.1	ParA family protein	-
BANH1_RS00310	70880..71173	+	294	WP_041582858.1	hypothetical protein	-
BANH1_RS00315	71288..71620	-	333	WP_015397462.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BANH1_RS00320	71620..71877	-	258	WP_015397463.1	PemI protein	Antitoxin
BANH1_RS00325	72076..72720	-	645	WP_041582860.1	recombinase family protein	-
BANH1_RS00330	72935..73225	+	291	WP_015397465.1	YacA protein	-
BANH1_RS00335	73312..73674	-	363	WP_051039086.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BANH1_RS00340	73932..75002	+	1071	WP_015397466.1	heat-inducible transcriptional repressor HrcA	-
BANH1_RS00345	75123..75797	+	675	WP_015397467.1	nucleotide exchange factor GrpE	-
BANH1_RS00350	75880..76419	-	540	WP_015397468.1	RNA 2',3'-cyclic phosphodiesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11751.57 Da
• Isoelectric Point: 10.9383

>T28969 WP_015397462.1 NC_020300:c71620-71288 [Bartonella australis Aust/NH1]
MERGDIFLVSLNPTSGHEQQGTRPVLIVSPGAFNRLTKTSIVLPITSGSNFARTAGFTVSLMRAGTQTTGVVRCDQPRAL
DIGSRRGRKLESVPAAVIDEVMAKLATILE

Nucleotide

• Length: 333 bp

>T28969 NC_020300:c71620-71288 [Bartonella australis Aust/NH1]
ATGGAGCGAGGCGATATCTTTCTGGTGTCTCTCAACCCCACATCAGGCCATGAACAACAAGGCACGCGCCCGGTGCTGAT
CGTGTCGCCGGGTGCGTTTAACCGTCTGACGAAAACCTCAATCGTTCTGCCGATCACAAGCGGGAGCAACTTTGCCCGGA
CTGCAGGCTTTACCGTCTCCCTCATGAGAGCAGGGACTCAAACAACAGGAGTTGTGCGCTGCGATCAACCTAGAGCGTTG
GACATTGGATCTCGTCGGGGTCGTAAACTCGAAAGCGTGCCCGCTGCGGTCATTGATGAAGTTATGGCGAAACTCGCCAC
CATTCTTGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9193.71 Da
• Isoelectric Point: 5.0491

>AT28969 WP_015397463.1 NC_020300:c71877-71620 [Bartonella australis Aust/NH1]
MHTTTLRKVGGSVMLAVPPALLDVLRLQPGAKVGMAVDGRKLIIEPQARARYTLDELLAQCDPSAELTAEDREWLDSKPV
GGELL

Nucleotide

• Length: 258 bp

>AT28969 NC_020300:c71877-71620 [Bartonella australis Aust/NH1]
ATGCACACCACAACTCTGCGCAAGGTCGGCGGGTCAGTCATGCTGGCTGTTCCTCCTGCTCTGCTTGATGTGCTCCGCCT
TCAGCCGGGGGCCAAGGTCGGGATGGCCGTTGATGGCAGAAAACTCATTATCGAGCCACAAGCCCGCGCACGCTACACGC
TCGATGAGCTGCTTGCTCAATGTGACCCTTCTGCCGAGCTGACCGCCGAAGATCGGGAGTGGCTGGATTCCAAGCCCGTT
GGTGGTGAGCTGCTGTAA

Structures

Toxin

• AlphaFold DB: M1NRJ6

Antitoxin

• AlphaFold DB: M1N227