Detailed information of TA system TA028968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 67460..68007
• Replicon: chromosome
• Accession: NC_020300
• Organism: Bartonella australis Aust/NH1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M1PBC3
• Locus tag: BANH1_RS00275
• Protein ID: WP_015397455.1
• Coordinates: 67460..67783 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BANH1_RS00280
• Protein ID: WP_019224016.1
• Coordinates: 67783..68007 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BANH1_RS00240	62763..63962	-	1200	WP_015397448.1	coproporphyrinogen III oxidase	-
BANH1_RS00245	63962..64609	-	648	WP_015397449.1	non-canonical purine NTP pyrophosphatase	-
BANH1_RS00250	64859..65170	-	312	WP_015397450.1	hypothetical protein	-
BANH1_RS00255	65167..65535	-	369	WP_015397451.1	conjugal transfer transcriptional regulator TraJ	-
BANH1_RS00260	65789..66055	+	267	WP_144050527.1	hypothetical protein	-
BANH1_RS00265	66186..66917	+	732	WP_015397453.1	conjugal transfer pilus assembly protein TraL	-
BANH1_RS00270	66929..67387	+	459	WP_015397454.1	conjugal transfer protein TraM	-
BANH1_RS00275	67460..67783	-	324	WP_015397455.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BANH1_RS00280	67783..68007	-	225	WP_019224016.1	antitoxin MazE family protein	Antitoxin
BANH1_RS00285	68151..68402	+	252	WP_015397457.1	hypothetical protein	-
BANH1_RS00290	68399..68797	+	399	WP_015397458.1	type II toxin-antitoxin system VapC family toxin	-
BANH1_RS00295	69084..69602	+	519	WP_015397459.1	replication/maintenance protein RepL	-
BANH1_RS00300	69762..70019	-	258	WP_015397460.1	hypothetical protein	-
BANH1_RS00305	70016..70666	-	651	WP_015397461.1	ParA family protein	-
BANH1_RS00310	70880..71173	+	294	WP_041582858.1	hypothetical protein	-
BANH1_RS00315	71288..71620	-	333	WP_015397462.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BANH1_RS00320	71620..71877	-	258	WP_015397463.1	PemI protein	-
BANH1_RS00325	72076..72720	-	645	WP_041582860.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-102)

Antitoxin

• Antitoxin MazE-like (7-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11731.83 Da
• Isoelectric Point: 8.5556

>T28968 WP_015397455.1 NC_020300:c67783-67460 [Bartonella australis Aust/NH1]
MRGSFVTIAMQGDFGKPRPALIIQADQFSEHTSVTVLPITSTLVAAPLLRITLQPDTQNGLQKTSQVMIDKTMTVRCEKV
SPAFGFVHADKMVEIERCLAVFLGIVK

Nucleotide

• Length: 324 bp

>T28968 NC_020300:c67783-67460 [Bartonella australis Aust/NH1]
ATGCGTGGATCTTTCGTAACAATAGCTATGCAAGGCGATTTTGGTAAACCAAGACCTGCGTTAATCATTCAAGCCGATCA
ATTTAGCGAACATACAAGCGTAACGGTTTTACCCATTACCAGCACACTTGTTGCGGCGCCATTACTGCGCATTACTCTCC
AACCAGATACCCAAAATGGTTTACAAAAAACTTCTCAGGTCATGATTGATAAGACTATGACGGTAAGATGTGAAAAAGTT
AGCCCAGCTTTTGGCTTCGTTCATGCAGATAAAATGGTGGAAATTGAACGCTGTTTAGCTGTATTTTTAGGAATAGTAAA
ATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8776.13 Da
• Isoelectric Point: 8.5634

>AT28968 WP_019224016.1 NC_020300:c68007-67783 [Bartonella australis Aust/NH1]
MATMHVNERVQKHRNAQRKAGLRLMQIWVPDTRQPNFAEECRRQCRLVAKMDKTDTSMQLLMDQSLMDVDGWTE

Nucleotide

• Length: 225 bp

>AT28968 NC_020300:c68007-67783 [Bartonella australis Aust/NH1]
ATGGCTACAATGCACGTCAATGAACGCGTTCAAAAACATCGCAACGCGCAGAGAAAAGCCGGATTGCGCTTAATGCAAAT
TTGGGTACCAGATACTCGCCAACCGAACTTTGCAGAAGAGTGTCGACGCCAATGCCGCTTAGTGGCAAAAATGGATAAAA
CAGACACATCTATGCAGTTGTTAATGGATCAATCTTTAATGGATGTTGATGGCTGGACAGAATGA

Structures

Toxin

• AlphaFold DB: M1PBC3