289620

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 3915073..3916793 Replicon chromosome
Accession NZ_LR882057
Organism Escherichia coli isolate 2014-01-7375

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag IHP09_RS18990 Protein ID WP_000312833.1
Coordinates 3915073..3915729 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag IHP09_RS18995 Protein ID WP_000634203.1
Coordinates 3915726..3916793 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHP09_RS18975 3910988..3911227 + 240 WP_024224214.1 hypothetical protein -
IHP09_RS18980 3911267..3913705 + 2439 WP_032180025.1 type I restriction-modification system subunit M -
IHP09_RS18985 3913717..3915060 + 1344 WP_134888771.1 restriction endonuclease subunit S -
IHP09_RS18990 3915073..3915729 + 657 WP_000312833.1 DUF4433 domain-containing protein Toxin
IHP09_RS18995 3915726..3916793 + 1068 WP_000634203.1 macro domain-containing protein Antitoxin
IHP09_RS19000 3916812..3919907 + 3096 WP_059337602.1 HsdR family type I site-specific deoxyribonuclease -
IHP09_RS19005 3919907..3920623 + 717 WP_001122107.1 M48 family metallopeptidase -
IHP09_RS19010 3921477..3921731 - 255 WP_001222075.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3870955..3943414 72459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T289620 WP_000312833.1 NZ_LR882057:3915073-3915729 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT289620 WP_000634203.1 NZ_LR882057:3915726-3916793 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References