Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 291386..292186 Replicon chromosome
Accession NZ_LR882057
Organism Escherichia coli isolate 2014-01-7375

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag IHP09_RS01335 Protein ID WP_000342449.1
Coordinates 291659..292186 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag IHP09_RS01330 Protein ID WP_001277108.1
Coordinates 291386..291652 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHP09_RS01310 287044..287712 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
IHP09_RS01315 287705..288763 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
IHP09_RS01320 289008..289862 + 855 WP_000130221.1 RNA polymerase sigma factor RpoH -
IHP09_RS01325 290133..291236 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
IHP09_RS01330 291386..291652 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
IHP09_RS01335 291659..292186 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
IHP09_RS01340 292183..292566 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
IHP09_RS01345 292990..294099 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
IHP09_RS01350 294147..295073 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IHP09_RS01355 295070..296347 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
IHP09_RS01360 296344..297111 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T289604 WP_000342449.1 NZ_LR882057:291659-292186 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT289604 WP_001277108.1 NZ_LR882057:291386-291652 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References