Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 93739..94264 Replicon plasmid 2
Accession NZ_LR882053
Organism Escherichia coli isolate 2015-01-466

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag QREC_RS22390 Protein ID WP_001159868.1
Coordinates 93739..94044 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag QREC_RS22395 Protein ID WP_000813634.1
Coordinates 94046..94264 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QREC_RS22375 89649..90815 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
QREC_RS22380 91403..92158 - 756 WP_000852146.1 replication initiation protein RepE -
QREC_RS22385 92932..93738 - 807 WP_000016982.1 site-specific integrase -
QREC_RS22390 93739..94044 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
QREC_RS22395 94046..94264 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QREC_RS22400 94972..95967 + 996 WP_000246636.1 hypothetical protein -
QREC_RS22405 95971..96903 + 933 WP_058682609.1 hypothetical protein -
QREC_RS22410 97103..97800 - 698 Protein_121 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / aac(3)-IIa / mph(A) / sul1 / aadA5 - 1..113096 113096


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T289598 WP_001159868.1 NZ_LR882053:c94044-93739 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT289598 WP_000813634.1 NZ_LR882053:c94264-94046 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References