Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3855135..3855711 Replicon chromosome
Accession NZ_LR882052
Organism Escherichia coli isolate 2015-01-466

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QREC_RS18440 Protein ID WP_001297643.1
Coordinates 3855424..3855711 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QREC_RS18435 Protein ID WP_000063156.1
Coordinates 3855135..3855437 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QREC_RS18420 3851731..3853881 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QREC_RS18425 3853931..3854134 + 204 WP_000467859.1 YbdD/YjiX family protein -
QREC_RS18430 3854145..3855101 + 957 WP_001297640.1 GTPase -
QREC_RS18435 3855135..3855437 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QREC_RS18440 3855424..3855711 - 288 WP_001297643.1 BrnT family toxin Toxin
QREC_RS18445 3855910..3856607 - 698 Protein_3599 IS1 family transposase -
QREC_RS18450 3856664..3856759 + 96 Protein_3600 dienelactone hydrolase family protein -
QREC_RS18455 3856905..3858137 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
QREC_RS18460 3858178..3859458 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3855910..3856119 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T289595 WP_001297643.1 NZ_LR882052:c3855711-3855424 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT289595 WP_000063156.1 NZ_LR882052:c3855437-3855135 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References