Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3971772..3972426 Replicon chromosome
Accession NZ_LR882050
Organism Escherichia coli isolate 2016-02-324

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IHP20_RS19125 Protein ID WP_000244777.1
Coordinates 3972019..3972426 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IHP20_RS19120 Protein ID WP_000354046.1
Coordinates 3971772..3972038 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHP20_RS19095 3966941..3967684 + 744 WP_000951948.1 SDR family oxidoreductase -
IHP20_RS19100 3967741..3969174 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
IHP20_RS19105 3969219..3969530 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IHP20_RS19110 3969694..3970353 + 660 WP_000250274.1 hemolysin III family protein -
IHP20_RS19115 3970549..3971529 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IHP20_RS19120 3971772..3972038 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IHP20_RS19125 3972019..3972426 + 408 WP_000244777.1 toxin CptA Toxin
IHP20_RS19130 3972466..3972987 - 522 WP_001055874.1 flavodoxin FldB -
IHP20_RS19135 3973099..3973995 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IHP20_RS19140 3974020..3974730 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IHP20_RS19145 3974736..3976469 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T289576 WP_000244777.1 NZ_LR882050:3972019-3972426 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT289576 WP_000354046.1 NZ_LR882050:3971772-3972038 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References