Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2638833..2639330 Replicon chromosome
Accession NZ_LR882050
Organism Escherichia coli isolate 2016-02-324

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IHP20_RS12840 Protein ID WP_001173343.1
Coordinates 2638833..2639006 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IHP20_RS12845 Protein ID WP_000398619.1
Coordinates 2639034..2639330 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHP20_RS12830 2635498..2636955 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IHP20_RS12835 2637192..2638709 + 1518 WP_001295074.1 lysine--tRNA ligase -
IHP20_RS12840 2638833..2639006 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IHP20_RS12845 2639034..2639330 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IHP20_RS12850 2639557..2639829 - 273 WP_000405647.1 N-acetyltransferase -
IHP20_RS12855 2639841..2640071 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
IHP20_RS12860 2640252..2641883 + 1632 WP_074503002.1 sensor histidine kinase -
IHP20_RS12865 2641880..2642599 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T289572 WP_001173343.1 NZ_LR882050:c2639006-2638833 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT289572 WP_000398619.1 NZ_LR882050:c2639330-2639034 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References