Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4192287..4192784 Replicon chromosome
Accession NZ_LR881940
Organism Escherichia coli isolate 2015-01-2097

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IHP11_RS20330 Protein ID WP_001173343.1
Coordinates 4192287..4192460 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IHP11_RS20335 Protein ID WP_000398619.1
Coordinates 4192488..4192784 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHP11_RS20320 4188957..4190414 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IHP11_RS20325 4190651..4192168 + 1518 WP_001295074.1 lysine--tRNA ligase -
IHP11_RS20330 4192287..4192460 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IHP11_RS20335 4192488..4192784 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IHP11_RS20340 4193011..4193283 - 273 WP_000405647.1 N-acetyltransferase -
IHP11_RS20345 4193295..4193525 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
IHP11_RS20350 4193706..4195337 + 1632 WP_001216477.1 sensor histidine kinase -
IHP11_RS20355 4195334..4196053 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T289555 WP_001173343.1 NZ_LR881940:c4192460-4192287 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT289555 WP_000398619.1 NZ_LR881940:c4192784-4192488 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References