Detailed information of TA system TA028954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3460105..3460742
• Replicon: chromosome
• Accession: NC_020272
• Organism: Bacillus amyloliquefaciens IT-45

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KSO_RS16745
• Protein ID: WP_003156187.1
• Coordinates: 3460105..3460455 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KSO_RS16750
• Protein ID: WP_003156188.1
• Coordinates: 3460461..3460742 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSO_RS16705	3455145..3455747	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
KSO_RS16710	3455747..3456535	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
KSO_RS16715	3456501..3456983	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
KSO_RS16720	3456980..3457309	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
KSO_RS16725	3457373..3458380	-	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
KSO_RS16730	3458392..3458793	-	402	WP_003156178.1	anti-sigma regulatory factor	-
KSO_RS16735	3458796..3459161	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
KSO_RS16740	3459166..3459987	-	822	WP_003156182.1	STAS domain-containing protein	-
KSO_RS16745	3460105..3460455	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KSO_RS16750	3460461..3460742	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KSO_RS16755	3460862..3462031	-	1170	WP_015388675.1	alanine racemase	-
KSO_RS16760	3462148..3463155	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
KSO_RS16765	3463320..3463685	-	366	WP_014304402.1	holo-ACP synthase	-
KSO_RS16770	3463778..3464377	+	600	WP_015388676.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T28954 WP_003156187.1 NC_020272:c3460455-3460105 [Bacillus amyloliquefaciens IT-45]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T28954 NC_020272:c3460455-3460105 [Bacillus amyloliquefaciens IT-45]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT28954 WP_003156188.1 NC_020272:c3460742-3460461 [Bacillus amyloliquefaciens IT-45]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT28954 NC_020272:c3460742-3460461 [Bacillus amyloliquefaciens IT-45]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5