Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2894247..2894830 Replicon chromosome
Accession NZ_LR881938
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag IEU92_RS14105 Protein ID WP_000254738.1
Coordinates 2894247..2894582 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IEU92_RS14110 Protein ID WP_000581937.1
Coordinates 2894582..2894830 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEU92_RS14090 2890134..2891432 - 1299 WP_000036723.1 phosphopyruvate hydratase -
IEU92_RS14095 2891520..2893157 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IEU92_RS14100 2893385..2894176 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IEU92_RS14105 2894247..2894582 - 336 WP_000254738.1 endoribonuclease MazF Toxin
IEU92_RS14110 2894582..2894830 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IEU92_RS14115 2894908..2897142 - 2235 WP_000226815.1 GTP pyrophosphokinase -
IEU92_RS14120 2897190..2898491 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T289523 WP_000254738.1 NZ_LR881938:c2894582-2894247 [Escherichia coli str. K-12 substr. MG1655]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT289523 WP_000581937.1 NZ_LR881938:c2894830-2894582 [Escherichia coli str. K-12 substr. MG1655]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References