Detailed information of TA system TA028950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 38271..38914
• Replicon: plasmid pSP291-2
• Accession: NC_020261
• Organism: Cronobacter sakazakii SP291

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CSSP291_RS20985
• Protein ID: WP_015387203.1
• Coordinates: 38271..38687 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CSSP291_RS20990
• Protein ID: WP_015387204.1
• Coordinates: 38684..38914 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSSP291_RS20955	33823..34062	-	240	WP_012815885.1	hypothetical protein	-
CSSP291_RS20960	34115..34732	-	618	WP_015387198.1	ParA family protein	-
CSSP291_RS20965	35607..36014	-	408	WP_015387199.1	glutathione S-transferase C-terminal domain-containing protein	-
CSSP291_RS20970	36405..37175	+	771	WP_015387200.1	DUF4225 domain-containing protein	-
CSSP291_RS20975	37179..37502	-	324	WP_015387201.1	hypothetical protein	-
CSSP291_RS20980	37559..37852	-	294	WP_015387202.1	hypothetical protein	-
CSSP291_RS20985	38271..38687	-	417	WP_015387203.1	type II toxin-antitoxin system VapC family toxin	Toxin
CSSP291_RS20990	38684..38914	-	231	WP_015387204.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CSSP291_RS22195	39362..39520	+	159	WP_015387206.1	type I toxin-antitoxin system Hok family toxin	-
CSSP291_RS22935	41167..41473	+	307	Protein_40	protein TraE	-
CSSP291_RS21000	41427..41825	+	399	Protein_41	thioredoxin domain-containing protein	-
CSSP291_RS21005	42494..43197	+	704	Protein_42	hypothetical protein	-
CSSP291_RS21010	43341..43634	-	294	WP_007853370.1	DUF1493 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52134	52134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14924.31 Da
• Isoelectric Point: 7.2449

>T28950 WP_015387203.1 NC_020261:c38687-38271 [Cronobacter sakazakii SP291]
VIKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGLLVEAFCARLDAILAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWAN

Nucleotide

• Length: 417 bp

>T28950 NC_020261:c38687-38271 [Cronobacter sakazakii SP291]
GTGATCAAGACCTATATGTTGGACACCAACATTTGCTCATTCATTATGCGTGAGCAACCGGAAGCTGTCATCAAACGGCT
GGAACAGGCAGTACTGCGCAACCACCGCATTGTGGTTTCGGCCATTACCTATGCCGAGATGCGTTTCGGTGCGATCGGAA
AGAAGGCATCTCCCCGGCACGGCCTGCTTGTCGAAGCTTTCTGCGCCCGCCTCGATGCAATCCTCGCCTGGGACCGCGCC
GCAGTAGACGCAACCACAGAGATTAAGGCTGCGCTGACTGCGGCCGGGACGCCAATCGGTCCGAACGACACGGCGATTGC
CGGGCATGCTATCGCCGCCGGCGCCGTACTAGTGACGAACAACGTGCGAGAGTTTGAGCGTGTGCCTGGACTGGTTCTGG
AAGACTGGGCTAACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.6182

>AT28950 WP_015387204.1 NC_020261:c38914-38684 [Cronobacter sakazakii SP291]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFRQLEKADPDFMAERGDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT28950 NC_020261:c38914-38684 [Cronobacter sakazakii SP291]
ATGAGAACCGTATCGATATTTAAAAACGGCAATAATCGTGCTATCCGTCTGCCCCGAGACCTGGATTTTGAGGGTGTGAG
TGAACTGGAGATCGTCCGGGAAGGTGACAGCATCATTCTTCGTCCCGTTCGCCCAACATGGGGTTCGTTCAGGCAACTCG
AAAAAGCCGATCCGGACTTTATGGCCGAACGTGGGGACGTTGTCAGCGATGAAGGACGATTTGACCTGTGA