Detailed information of TA system TA028949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2803966..2804585
• Replicon: chromosome
• Accession: NC_020260
• Organism: Cronobacter sakazakii SP291

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A2S9UIC9
• Locus tag: CSSP291_RS13285
• Protein ID: WP_004386909.1
• Coordinates: 2804367..2804585 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CSSP291_RS13280
• Protein ID: WP_015386942.1
• Coordinates: 2803966..2804340 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSSP291_RS13270	2799127..2800332	+	1206	WP_007848022.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CSSP291_RS13275	2800354..2803503	+	3150	WP_007848020.1	multidrug efflux RND transporter permease subunit	-
CSSP291_RS13280	2803966..2804340	+	375	WP_015386942.1	Hha toxicity modulator TomB	Antitoxin
CSSP291_RS13285	2804367..2804585	+	219	WP_004386909.1	hemolysin expression modulator Hha	Toxin
CSSP291_RS13290	2804794..2805345	+	552	WP_004386910.1	maltose O-acetyltransferase	-
CSSP291_RS13295	2805465..2805974	+	510	WP_007891920.1	YlaC family protein	-
CSSP291_RS21895	2806003..2806143	-	141	WP_004386911.1	type B 50S ribosomal protein L36	-
CSSP291_RS13300	2806147..2806407	-	261	WP_004386912.1	type B 50S ribosomal protein L31	-
CSSP291_RS13305	2806548..2808449	-	1902	WP_014729414.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8598.02 Da
• Isoelectric Point: 9.4828

>T28949 WP_004386909.1 NC_020260:2804367-2804585 [Cronobacter sakazakii SP291]
MTTKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 219 bp

>T28949 NC_020260:2804367-2804585 [Cronobacter sakazakii SP291]
ATGACGACCAAACCATTAACCAAAACCGATTATTTAATGCGACTGAGACGTTGTCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTATCCGACAACGAACTGGCGGTATTTTATTCAGCGGCGGATCATCGTCTGGCTGAAC
TGACGATGAATAAGCTCTACGATAAAATTCCGACCGCCGTGTGGAAGTTCGTTCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14315.00 Da
• Isoelectric Point: 4.7168

>AT28949 WP_015386942.1 NC_020260:2803966-2804340 [Cronobacter sakazakii SP291]
MDEYSPKRHDIAQLKFLCESLYHDCLATLGENQHGWANDPTSAVNLQLNDLIEHIASFGLNYKIKHEDDAALIEQLDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFVNASKENPVSLSF

Nucleotide

• Length: 375 bp

>AT28949 NC_020260:2803966-2804340 [Cronobacter sakazakii SP291]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTTAAATTTCTTTGCGAAAGCCTGTATCATGATTGCCTTGC
CACGCTCGGTGAAAACCAGCATGGCTGGGCTAACGACCCCACTTCTGCCGTTAACCTGCAACTCAACGACTTGATTGAGC
ATATTGCCAGCTTCGGCCTGAATTATAAAATTAAGCATGAAGATGACGCAGCTCTCATTGAGCAATTAGATGAATATCTG
GATGATACCTTTATGCTGTTCAGCAATTACGGTATCAATGCGCAAGATTTACAAAAATGGCGCAAGTCCGGAAACAGGCT
TTTCCGGTGTTTCGTAAACGCCAGCAAAGAAAACCCTGTAAGCTTGTCTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A2S9UIC9