Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 238887..239413 Replicon plasmid pPSV
Accession NZ_LR881935
Organism Citrobacter freundii strain PSV

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CITRO_RS26590 Protein ID WP_000323025.1
Coordinates 238887..239174 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CITRO_RS26595 Protein ID WP_000534858.1
Coordinates 239174..239413 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CITRO_RS26535 234221..234604 + 384 WP_050596975.1 hypothetical protein -
CITRO_RS26540 234615..234869 + 255 WP_032610479.1 hypothetical protein -
CITRO_RS26545 234869..235285 + 417 WP_032610480.1 hypothetical protein -
CITRO_RS26550 235403..235684 + 282 WP_032610524.1 hypothetical protein -
CITRO_RS26555 235870..236424 + 555 WP_015063016.1 hypothetical protein -
CITRO_RS26560 236484..236732 + 249 WP_050596976.1 hypothetical protein -
CITRO_RS26565 236857..237189 + 333 WP_032610481.1 DUF5417 domain-containing protein -
CITRO_RS26570 237234..237803 + 570 WP_032610483.1 hypothetical protein -
CITRO_RS26575 237827..238177 + 351 WP_032610485.1 hypothetical protein -
CITRO_RS26580 238217..238423 + 207 WP_044255297.1 hypothetical protein -
CITRO_RS26585 238658..238816 - 159 WP_064758473.1 type I toxin-antitoxin system Hok family toxin -
CITRO_RS26590 238887..239174 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CITRO_RS26595 239174..239413 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CITRO_RS26600 239676..240599 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
CITRO_RS26605 241071..242051 + 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
CITRO_RS26610 242200..242571 - 372 Protein_239 cytochrome b/b6 domain-containing protein -
CITRO_RS26615 243047..244285 - 1239 WP_003030308.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..295415 295415
- flank IS/Tn - - 241071..242051 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T289485 WP_000323025.1 NZ_LR881935:c239174-238887 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT289485 WP_000534858.1 NZ_LR881935:c239413-239174 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References