Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 73938..75561 Replicon plasmid pPSV
Accession NZ_LR881935
Organism Citrobacter freundii strain PSV

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag CITRO_RS25760 Protein ID WP_202581372.1
Coordinates 74236..75561 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A7W3GWM9
Locus tag CITRO_RS25755 Protein ID WP_058656924.1
Coordinates 73938..74231 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CITRO_RS25730 69104..69283 + 180 WP_071685142.1 hypothetical protein -
CITRO_RS25735 69449..70105 + 657 WP_202581371.1 trypsin-like serine protease -
CITRO_RS25740 70308..70805 + 498 WP_000340139.1 membrane protein -
CITRO_RS25745 70863..72103 - 1241 Protein_66 IS3 family transposase -
CITRO_RS25750 72185..73537 + 1353 WP_058656920.1 hypothetical protein -
CITRO_RS25755 73938..74231 + 294 WP_058656924.1 helix-turn-helix transcriptional regulator Antitoxin
CITRO_RS25760 74236..75561 + 1326 WP_202581372.1 type II toxin-antitoxin system HipA family toxin Toxin
CITRO_RS25765 75622..75828 + 207 WP_000134171.1 hypothetical protein -
CITRO_RS25770 75998..76492 + 495 WP_071685143.1 hypothetical protein -
CITRO_RS25775 76492..78090 + 1599 WP_020833803.1 hypothetical protein -
CITRO_RS25780 78254..79156 + 903 WP_020833802.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..295415 295415
- flank IS/Tn - - 70863..71750 887


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50275.17 Da        Isoelectric Point: 6.0252

>T289484 WP_202581372.1 NZ_LR881935:74236-75561 [Citrobacter freundii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWVMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11024.41 Da        Isoelectric Point: 9.9492

>AT289484 WP_058656924.1 NZ_LR881935:73938-74231 [Citrobacter freundii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSATTSSERK
QTEEIYKFNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3GWM9

References