Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5051712..5052328 Replicon chromosome
Accession NZ_LR881934
Organism Citrobacter freundii strain PSV

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag CITRO_RS24560 Protein ID WP_003028682.1
Coordinates 5051712..5052086 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag CITRO_RS24565 Protein ID WP_043018956.1
Coordinates 5052086..5052328 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CITRO_RS24545 5049215..5050117 + 903 WP_016155183.1 formate dehydrogenase O subunit beta -
CITRO_RS24550 5050114..5050749 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
CITRO_RS24555 5050746..5051675 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
CITRO_RS24560 5051712..5052086 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
CITRO_RS24565 5052086..5052328 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
CITRO_RS24570 5052534..5053441 + 908 Protein_4806 alpha/beta hydrolase -
CITRO_RS24575 5053592..5054533 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
CITRO_RS24580 5054578..5055015 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
CITRO_RS24585 5055012..5055884 - 873 WP_003028669.1 virulence factor BrkB family protein -
CITRO_RS24590 5055878..5056477 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T289483 WP_003028682.1 NZ_LR881934:c5052086-5051712 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT289483 WP_043018956.1 NZ_LR881934:c5052328-5052086 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References