Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 118023..118648 Replicon plasmid 83_VP
Accession NZ_LR878366
Organism Shigella flexneri 3a isolate 83

Toxin (Protein)


Gene name vapC Uniprot ID Q7BEJ1
Locus tag JMV05_RS23565 Protein ID WP_000911311.1
Coordinates 118250..118648 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q7BEJ0
Locus tag JMV05_RS23560 Protein ID WP_000450531.1
Coordinates 118023..118250 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV05_RS23555 114722..117941 - 3220 Protein_147 conjugative transfer relaxase/helicase TraI -
JMV05_RS23560 118023..118250 + 228 WP_000450531.1 toxin-antitoxin system antitoxin VapB Antitoxin
JMV05_RS23565 118250..118648 + 399 WP_000911311.1 type II toxin-antitoxin system VapC family toxin Toxin
JMV05_RS23570 118657..118836 - 180 Protein_150 hypothetical protein -
JMV05_RS23575 118881..120037 + 1157 WP_094099560.1 IS3-like element IS600 family transposase -
JMV05_RS23580 120082..121005 + 924 Protein_152 IS630-like element IS630 family transposase -
JMV05_RS23585 121159..122103 - 945 WP_004996485.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
JMV05_RS23590 122168..123118 - 951 WP_025760081.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospD3/senA / ospC1 / ipaH4.5 / ipaH7.8 / ospC2 / icsP/sopA / ospB / ospC3 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 1..231165 231165
- inside IScluster/Tn - - 107527..121005 13478


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14817.08 Da        Isoelectric Point: 8.5232

>T289458 WP_000911311.1 NZ_LR878366:118250-118648 [Shigella flexneri 3a]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8565.62 Da        Isoelectric Point: 4.7513

>AT289458 WP_000450531.1 NZ_LR878366:118023-118250 [Shigella flexneri 3a]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSTDFMDNREQPGMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3TND
PDB 5ED0
PDB 5ECW
PDB 5ECD
PDB 5ECY

Antitoxin

Source ID Structure
PDB 3TND
AlphaFold DB Q7BEJ0

References