Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1461514..1462298 Replicon chromosome
Accession NZ_LR778152
Organism Escherichia coli isolate SC477

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag H1D09_RS07025 Protein ID WP_000613626.1
Coordinates 1461804..1462298 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag H1D09_RS07020 Protein ID WP_001110446.1
Coordinates 1461514..1461807 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D09_RS07010 1456663..1457622 - 960 WP_000846343.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
H1D09_RS07015 1458195..1461380 + 3186 WP_097447887.1 ribonuclease E -
H1D09_RS07020 1461514..1461807 + 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
H1D09_RS07025 1461804..1462298 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
H1D09_RS07030 1462393..1463346 - 954 WP_001212764.1 flagellar hook-associated protein FlgL -
H1D09_RS07035 1463358..1465001 - 1644 WP_053898721.1 flagellar hook-associated protein FlgK -
H1D09_RS07040 1465067..1466008 - 942 WP_001337731.1 flagellar assembly peptidoglycan hydrolase FlgJ -
H1D09_RS07045 1466008..1467105 - 1098 WP_000589300.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T289286 WP_000613626.1 NZ_LR778152:1461804-1462298 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT289286 WP_001110446.1 NZ_LR778152:1461514-1461807 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References