Detailed information of TA system TA028928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 1764228..1764834
• Replicon: chromosome
• Accession: NC_020247
• Organism: Sulfolobus acidocaldarius Ron12/I

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1ITB5
• Locus tag: SACRON12I_RS09435
• Protein ID: WP_011278759.1
• Coordinates: 1764228..1764623 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I7B9
• Locus tag: SACRON12I_RS09440
• Protein ID: WP_011278760.1
• Coordinates: 1764607..1764834 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACRON12I_RS09410 (SacRon12I_09655)	1760580..1761110	+	531	WP_011278754.1	PaREP1 family protein	-
SACRON12I_RS09415 (SacRon12I_09660)	1761719..1762396	-	678	WP_011278755.1	hypothetical protein	-
SACRON12I_RS12330	1762995..1763120	-	126	WP_257719735.1	hypothetical protein	-
SACRON12I_RS09425 (SacRon12I_09665)	1763359..1763607	+	249	WP_011278757.1	hypothetical protein	-
SACRON12I_RS09430 (SacRon12I_09670)	1763600..1764019	+	420	WP_011278758.1	PIN domain-containing protein	-
SACRON12I_RS09435 (SacRon12I_09675)	1764228..1764623	-	396	WP_011278759.1	PIN domain-containing protein	Toxin
SACRON12I_RS09440 (SacRon12I_09680)	1764607..1764834	-	228	WP_011278760.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SACRON12I_RS09445 (SacRon12I_09685)	1764934..1765377	-	444	WP_011278761.1	MarR family transcriptional regulator	-
SACRON12I_RS09450 (SacRon12I_09690)	1765364..1767067	-	1704	WP_011278762.1	MFS transporter	-
SACRON12I_RS09455 (SacRon12I_09695)	1767644..1768141	+	498	WP_011278763.1	hypothetical protein	-
SACRON12I_RS09460 (SacRon12I_09700)	1768162..1768833	+	672	WP_015385737.1	hypothetical protein	-
SACRON12I_RS12080	1768839..1769606	+	768	WP_011278765.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (3-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15289.79 Da
• Isoelectric Point: 5.7030

>T28928 WP_011278759.1 NC_020247:c1764623-1764228 [Sulfolobus acidocaldarius Ron12/I]
MKILVDTNVLIYDLVENSKFHKEAEDILDRAETWVIPSVVVHELVWFLRANNLERMDYVSAYVESPKAEIVCEDFDVLKN
AMEIILREKISLSRYNDPVILSHAILRKLPLSTFDRRLSNLAKRYGIDIVK

Nucleotide

• Length: 396 bp

>T28928 NC_020247:c1764623-1764228 [Sulfolobus acidocaldarius Ron12/I]
ATGAAAATTTTAGTTGATACTAACGTACTAATCTATGACCTCGTGGAGAACTCAAAGTTTCACAAGGAAGCTGAGGATAT
ATTGGACAGAGCAGAAACTTGGGTCATACCTTCTGTAGTCGTTCATGAGCTAGTATGGTTCTTGAGGGCGAATAACCTGG
AGAGAATGGACTACGTATCAGCATATGTGGAAAGCCCCAAGGCTGAAATAGTGTGCGAGGACTTTGATGTTTTAAAGAAC
GCAATGGAAATAATACTGAGGGAAAAGATATCTCTTTCTAGGTATAATGACCCAGTAATACTCTCACACGCGATTCTCAG
GAAGTTACCACTTTCGACGTTTGATAGGAGACTCTCCAACTTGGCAAAGAGGTACGGAATAGACATAGTTAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8808.27 Da
• Isoelectric Point: 8.4634

>AT28928 WP_011278760.1 NC_020247:c1764834-1764607 [Sulfolobus acidocaldarius Ron12/I]
VEKVKVTRNYQVTIPSSVRNVLGIKEGDILEVYVENDKIVMKKVESKRPRIKIEKLMTIEYIEESIERGMHENFS

Nucleotide

• Length: 228 bp

>AT28928 NC_020247:c1764834-1764607 [Sulfolobus acidocaldarius Ron12/I]
GTGGAGAAGGTAAAAGTTACTAGGAACTACCAGGTCACGATACCTTCATCTGTGAGGAACGTCCTAGGAATTAAGGAGGG
TGATATTTTGGAGGTGTACGTAGAGAATGATAAAATAGTGATGAAGAAGGTAGAGTCTAAGAGACCTAGGATAAAGATTG
AAAAACTTATGACCATAGAATATATAGAGGAAAGTATAGAGAGAGGCATGCATGAAAATTTTAGTTGA

Structures

Toxin

• AlphaFold DB: M1ITB5

Antitoxin

• AlphaFold DB: M1I7B9