Detailed information of TA system TA028925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 1950759..1951741
• Replicon: chromosome
• Accession: NC_020246
• Organism: Sulfolobus acidocaldarius N8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IXW8
• Locus tag: SACN8_RS10265
• Protein ID: WP_011278959.1
• Coordinates: 1951121..1951741 (+)
• Length: 207 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I813
• Locus tag: SACN8_RS10260
• Protein ID: WP_011278958.1
• Coordinates: 1950759..1951109 (+)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACN8_RS10240 (SacN8_10520)	1946163..1948097	-	1935	WP_011278954.1	DUF2079 domain-containing protein	-
SACN8_RS10245 (SacN8_10525)	1948306..1949010	+	705	WP_011278955.1	zinc ribbon domain-containing protein	-
SACN8_RS10250 (SacN8_10530)	1949094..1949738	-	645	WP_011278956.1	2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase	-
SACN8_RS10255 (SacN8_10535)	1949785..1950480	-	696	WP_011278957.1	glycerophosphodiester phosphodiesterase	-
SACN8_RS10260 (SacN8_10540)	1950759..1951109	+	351	WP_011278958.1	DUF488 family protein	Antitoxin
SACN8_RS10265 (SacN8_10545)	1951121..1951741	+	621	WP_011278959.1	helix-turn-helix domain-containing protein	Toxin
SACN8_RS10270 (SacN8_10550)	1951846..1953252	-	1407	WP_015385784.1	amidase	-
SACN8_RS10275 (SacN8_10555)	1953456..1953905	+	450	WP_230937910.1	hypothetical protein	-
SACN8_RS10280 (SacN8_10560)	1953995..1955641	-	1647	WP_011278962.1	thermopsin family protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (147-197)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 207 a.a.
• Molecular weight: 23490.89 Da
• Isoelectric Point: 9.6857

>T28925 WP_011278959.1 NC_020246:1951121-1951741 [Sulfolobus acidocaldarius N8]
MGAGDVKKPINVTLILQNHPCLVMKLFEEMNVKAEINNVKMRESVTDHIATLKLTEKLLKELKERRSKTLRISENSVWIR
TEGCQVCKILYVSDAVVEKVKVVGSDAVMYKLIVPNLLSLKSLIDELNKIGVKAMVGSISELDEEQLTERQLEILKLSYK
MGFFDVDRRITMTELAEKLGIKAPTLEEILRRALRKAVKYYLDKKG

Nucleotide

• Length: 621 bp

>T28925 NC_020246:1951121-1951741 [Sulfolobus acidocaldarius N8]
ATGGGTGCTGGTGATGTAAAGAAACCTATAAATGTTACACTTATTCTACAGAATCACCCCTGCTTAGTTATGAAATTATT
TGAAGAGATGAATGTAAAGGCGGAAATTAACAATGTGAAGATGAGGGAAAGTGTTACTGACCACATTGCAACCTTAAAGT
TAACTGAAAAGTTGCTTAAGGAGTTGAAAGAAAGAAGGTCAAAAACCTTGAGGATAAGTGAAAATAGCGTCTGGATCAGG
ACTGAAGGTTGCCAGGTATGTAAAATCCTTTATGTAAGTGACGCTGTAGTTGAGAAAGTGAAAGTTGTAGGAAGTGATGC
AGTTATGTATAAACTAATTGTTCCAAATCTACTCTCTCTGAAGAGTCTTATAGATGAGCTCAACAAGATAGGTGTAAAGG
CTATGGTAGGTAGCATATCTGAGCTAGATGAAGAGCAACTGACAGAAAGGCAATTGGAGATATTGAAATTATCATATAAG
ATGGGTTTCTTTGATGTAGACAGAAGAATAACCATGACGGAATTAGCTGAAAAACTTGGGATCAAGGCTCCTACTTTGGA
AGAGATACTGAGAAGGGCTCTACGAAAGGCAGTTAAGTATTATCTAGATAAAAAAGGATAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13918.19 Da
• Isoelectric Point: 8.2649

>AT28925 WP_011278958.1 NC_020246:1950759-1951109 [Sulfolobus acidocaldarius N8]
MIKIKNVYDKPEINDGIRILVERTWPASVTRGSIDMWLRDLAPSEELITWYNYEPQKWEEFKRRYLEELRYNPKVKPLLM
IVKMTKIVTFLYSGDPTYNNAVVLKEYIESLINKES

Nucleotide

• Length: 351 bp

>AT28925 NC_020246:1950759-1951109 [Sulfolobus acidocaldarius N8]
ATGATAAAAATAAAAAACGTTTATGACAAACCAGAGATTAACGACGGGATTAGAATATTGGTCGAGAGGACATGGCCAGC
CTCTGTAACTAGAGGCTCTATCGATATGTGGCTTAGGGATCTTGCCCCTTCTGAAGAATTAATAACATGGTATAATTATG
AACCGCAAAAATGGGAGGAATTTAAGAGAAGATATCTAGAAGAGTTGAGGTACAATCCAAAAGTTAAACCTTTATTGATG
ATAGTTAAGATGACTAAGATCGTAACATTTCTATACTCAGGAGACCCCACATACAATAACGCAGTTGTGCTCAAAGAATA
CATAGAAAGTTTAATAAATAAGGAAAGTTAA

Structures

Toxin

• AlphaFold DB: M1IXW8

Antitoxin

• AlphaFold DB: M1I813