Detailed information of TA system TA028923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 1766040..1766646
• Replicon: chromosome
• Accession: NC_020246
• Organism: Sulfolobus acidocaldarius N8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1ITB5
• Locus tag: SACN8_RS09440
• Protein ID: WP_011278759.1
• Coordinates: 1766040..1766435 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I7B9
• Locus tag: SACN8_RS09445
• Protein ID: WP_011278760.1
• Coordinates: 1766419..1766646 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACN8_RS09415 (SacN8_09630)	1762392..1762922	+	531	WP_011278754.1	PaREP1 family protein	-
SACN8_RS09420 (SacN8_09635)	1763531..1764208	-	678	WP_011278755.1	hypothetical protein	-
SACN8_RS12095	1764807..1764932	-	126	WP_257719735.1	hypothetical protein	-
SACN8_RS09430 (SacN8_09640)	1765171..1765419	+	249	WP_011278757.1	hypothetical protein	-
SACN8_RS09435 (SacN8_09645)	1765412..1765831	+	420	WP_011278758.1	PIN domain-containing protein	-
SACN8_RS09440 (SacN8_09650)	1766040..1766435	-	396	WP_011278759.1	PIN domain-containing protein	Toxin
SACN8_RS09445 (SacN8_09655)	1766419..1766646	-	228	WP_011278760.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SACN8_RS09450 (SacN8_09660)	1766746..1767189	-	444	WP_011278761.1	MarR family transcriptional regulator	-
SACN8_RS09455 (SacN8_09665)	1767176..1768879	-	1704	WP_011278762.1	MFS transporter	-
SACN8_RS09460 (SacN8_09670)	1769456..1769953	+	498	WP_011278763.1	hypothetical protein	-
SACN8_RS09465 (SacN8_09675)	1769974..1770645	+	672	WP_015385737.1	hypothetical protein	-
SACN8_RS11840	1770651..1771418	+	768	WP_011278765.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (3-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15289.79 Da
• Isoelectric Point: 5.7030

>T28923 WP_011278759.1 NC_020246:c1766435-1766040 [Sulfolobus acidocaldarius N8]
MKILVDTNVLIYDLVENSKFHKEAEDILDRAETWVIPSVVVHELVWFLRANNLERMDYVSAYVESPKAEIVCEDFDVLKN
AMEIILREKISLSRYNDPVILSHAILRKLPLSTFDRRLSNLAKRYGIDIVK

Nucleotide

• Length: 396 bp

>T28923 NC_020246:c1766435-1766040 [Sulfolobus acidocaldarius N8]
ATGAAAATTTTAGTTGATACTAACGTACTAATCTATGACCTCGTGGAGAACTCAAAGTTTCACAAGGAAGCTGAGGATAT
ATTGGACAGAGCAGAAACTTGGGTCATACCTTCTGTAGTCGTTCATGAGCTAGTATGGTTCTTGAGGGCGAATAACCTGG
AGAGAATGGACTACGTATCAGCATATGTGGAAAGCCCCAAGGCTGAAATAGTGTGCGAGGACTTTGATGTTTTAAAGAAC
GCAATGGAAATAATACTGAGGGAAAAGATATCTCTTTCTAGGTATAATGACCCAGTAATACTCTCACACGCGATTCTCAG
GAAGTTACCACTTTCGACGTTTGATAGGAGACTCTCCAACTTGGCAAAGAGGTACGGAATAGACATAGTTAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8808.27 Da
• Isoelectric Point: 8.4634

>AT28923 WP_011278760.1 NC_020246:c1766646-1766419 [Sulfolobus acidocaldarius N8]
VEKVKVTRNYQVTIPSSVRNVLGIKEGDILEVYVENDKIVMKKVESKRPRIKIEKLMTIEYIEESIERGMHENFS

Nucleotide

• Length: 228 bp

>AT28923 NC_020246:c1766646-1766419 [Sulfolobus acidocaldarius N8]
GTGGAGAAGGTAAAAGTTACTAGGAACTACCAGGTCACGATACCTTCATCTGTGAGGAACGTCCTAGGAATTAAGGAGGG
TGATATTTTGGAGGTGTACGTAGAGAATGATAAAATAGTGATGAAGAAGGTAGAGTCTAAGAGACCTAGGATAAAGATTG
AAAAACTTATGACCATAGAATATATAGAGGAAAGTATAGAGAGAGGCATGCATGAAAATTTTAGTTGA

Structures

Toxin

• AlphaFold DB: M1ITB5

Antitoxin

• AlphaFold DB: M1I7B9