Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 292635..293289 Replicon chromosome
Accession NZ_LR778148
Organism Escherichia coli isolate SC475

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H1C74_RS01430 Protein ID WP_000244772.1
Coordinates 292635..293042 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H1C74_RS01435 Protein ID WP_000354046.1
Coordinates 293023..293289 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C74_RS01410 288592..290325 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
H1C74_RS01415 290331..291041 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H1C74_RS01420 291066..291962 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H1C74_RS01425 292074..292595 + 522 WP_001055874.1 flavodoxin FldB -
H1C74_RS01430 292635..293042 - 408 WP_000244772.1 protein YgfX Toxin
H1C74_RS01435 293023..293289 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H1C74_RS01440 293532..294512 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H1C74_RS01445 294589..295248 - 660 WP_000250274.1 hemolysin III family protein -
H1C74_RS01450 295412..295723 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
H1C74_RS01455 295768..297201 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
H1C74_RS01460 297258..298001 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T289211 WP_000244772.1 NZ_LR778148:c293042-292635 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT289211 WP_000354046.1 NZ_LR778148:c293289-293023 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References