Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2626536..2627119 Replicon chromosome
Accession NZ_LR778143
Organism Escherichia coli isolate SC422

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag H1C98_RS12450 Protein ID WP_000254745.1
Coordinates 2626784..2627119 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag H1C98_RS12445 Protein ID WP_000581937.1
Coordinates 2626536..2626784 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C98_RS12435 2622875..2624176 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
H1C98_RS12440 2624224..2626458 + 2235 WP_000226815.1 GTP pyrophosphokinase -
H1C98_RS12445 2626536..2626784 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
H1C98_RS12450 2626784..2627119 + 336 WP_000254745.1 endoribonuclease MazF Toxin
H1C98_RS12455 2627190..2627981 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
H1C98_RS12460 2628209..2629847 + 1639 Protein_2428 CTP synthase (glutamine hydrolyzing) -
H1C98_RS12465 2629935..2631233 + 1299 WP_000036723.1 phosphopyruvate hydratase -
H1C98_RS12470 2631289..2631651 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T289143 WP_000254745.1 NZ_LR778143:2626784-2627119 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT289143 WP_000581937.1 NZ_LR778143:2626536-2626784 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References