Detailed information of TA system TA028914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3060254..3060858
• Replicon: chromosome
• Accession: NC_020245
• Organism: Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: K60_RS14500
• Protein ID: WP_003414492.1
• Coordinates: 3060254..3060646 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: K60_RS14505
• Protein ID: WP_003414495.1
• Coordinates: 3060643..3060858 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K60_RS14470	3055404..3056192	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
K60_RS14475	3056526..3057071	-	546	WP_003904931.1	DUF1802 family protein	-
K60_RS14480	3057343..3058227	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
K60_RS14485	3058230..3059117	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
K60_RS14490	3059422..3059967	-	546	WP_003899500.1	DUF1802 family protein	-
K60_RS14495	3059964..3060233	-	270	WP_003414489.1	DUF2277 family protein	-
K60_RS14500	3060254..3060646	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
K60_RS14505	3060643..3060858	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K60_RS14510	3060905..3061654	+	750	WP_003414497.1	enoyl-CoA hydratase	-
K60_RS14515	3061733..3062815	-	1083	WP_011799293.1	ABC transporter ATP-binding protein	-
K60_RS21395	3062808..3064118	-	1311	Protein_2830	ABC transporter substrate-binding protein	-
K60_RS14530	3064121..3064948	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
K60_RS14535	3064945..3065856	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T28914 WP_003414492.1 NC_020245:c3060646-3060254 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T28914 NC_020245:c3060646-3060254 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT28914 WP_003414495.1 NC_020245:c3060858-3060643 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT28914 NC_020245:c3060858-3060643 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8