Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1021239..1021736 Replicon chromosome
Accession NZ_LR778143
Organism Escherichia coli isolate SC422

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H1C98_RS04955 Protein ID WP_001173343.1
Coordinates 1021239..1021412 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H1C98_RS04960 Protein ID WP_000398619.1
Coordinates 1021440..1021736 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C98_RS04945 1017909..1019366 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H1C98_RS04950 1019603..1021120 + 1518 WP_001295074.1 lysine--tRNA ligase -
H1C98_RS04955 1021239..1021412 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H1C98_RS04960 1021440..1021736 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H1C98_RS04965 1021963..1022235 - 273 WP_000405647.1 N-acetyltransferase -
H1C98_RS04970 1022247..1022477 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
H1C98_RS04975 1022658..1024289 + 1632 WP_001216461.1 sensor histidine kinase -
H1C98_RS04980 1024286..1025005 + 720 WP_000611296.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T289136 WP_001173343.1 NZ_LR778143:c1021412-1021239 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT289136 WP_000398619.1 NZ_LR778143:c1021736-1021440 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References