Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4030130..4030761 Replicon chromosome
Accession NZ_LR778142
Organism Escherichia coli isolate SC434

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag H1D10_RS19690 Protein ID WP_001260301.1
Coordinates 4030486..4030761 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H1D10_RS19685 Protein ID WP_000593555.1
Coordinates 4030130..4030489 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D10_RS19660 4026262..4027206 + 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
H1D10_RS19665 4027203..4028036 + 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
H1D10_RS19670 4028036..4028800 + 765 WP_001136232.1 nickel import ATP-binding protein NikD -
H1D10_RS19675 4028797..4029603 + 807 WP_000173679.1 nickel import ATP-binding protein NikE -
H1D10_RS19680 4029609..4030010 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H1D10_RS19685 4030130..4030489 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H1D10_RS19690 4030486..4030761 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
H1D10_RS19695 4030834..4031958 - 1125 WP_024231700.1 ABC transporter permease -
H1D10_RS19700 4031958..4034693 - 2736 WP_074448880.1 ribosome-associated ATPase/putative transporter RbbA -
H1D10_RS19705 4034690..4035757 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T289127 WP_001260301.1 NZ_LR778142:c4030761-4030486 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT289127 WP_000593555.1 NZ_LR778142:c4030489-4030130 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References