Detailed information of TA system TA028912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3033263..3033833
• Replicon: chromosome
• Accession: NC_020245
• Organism: Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: K60_RS14350
• Protein ID: WP_003414166.1
• Coordinates: 3033263..3033619 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: K60_RS14355
• Protein ID: WP_003901465.1
• Coordinates: 3033603..3033833 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K60_RS14330	3028715..3030403	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
K60_RS14335	3030407..3030733	-	327	WP_003414157.1	hypothetical protein	-
K60_RS14340	3030906..3031493	+	588	WP_003914429.1	DUF3558 family protein	-
K60_RS14345	3031512..3033161	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
K60_RS14350	3033263..3033619	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
K60_RS14355	3033603..3033833	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
K60_RS14360	3033876..3034919	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
K60_RS22045	3035110..3035385	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
K60_RS22050	3035561..3035815	-	255	WP_003917684.1	hypothetical protein	-
K60_RS14375	3035963..3036367	+	405	WP_003414181.1	hypothetical protein	-
K60_RS14380	3036364..3036555	+	192	WP_003414184.1	hypothetical protein	-
K60_RS14385	3036619..3037908	+	1290	Protein_2804	transposase family protein	-
K60_RS14390	3038142..3038399	+	258	WP_003899489.1	hypothetical protein	-
K60_RS14395	3038504..3038815	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T28912 WP_003414166.1 NC_020245:c3033619-3033263 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T28912 NC_020245:c3033619-3033263 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT28912 WP_003901465.1 NC_020245:c3033833-3033603 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT28912 NC_020245:c3033833-3033603 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3