Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4958032..4958869 Replicon chromosome
Accession NZ_LR778141
Organism Escherichia coli isolate SC407

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag H1C92_RS23975 Protein ID WP_000227784.1
Coordinates 4958032..4958574 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag H1C92_RS23980 Protein ID WP_001297137.1
Coordinates 4958558..4958869 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C92_RS23955 4953570..4954481 - 912 WP_000705847.1 2-dehydropantoate 2-reductase -
H1C92_RS23960 4954649..4955141 + 493 Protein_4672 nucleotide binding protein YajQ -
H1C92_RS23965 4955269..4956633 - 1365 WP_001000978.1 MFS transporter -
H1C92_RS23970 4957041..4957976 + 936 WP_001297127.1 sel1 repeat family protein -
H1C92_RS23975 4958032..4958574 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
H1C92_RS23980 4958558..4958869 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
H1C92_RS23985 4959054..4959944 - 891 WP_000971336.1 heme o synthase -
H1C92_RS23990 4959956..4960285 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
H1C92_RS23995 4960285..4960899 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
H1C92_RS24000 4960889..4962880 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
H1C92_RS24005 4962902..4963849 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T289109 WP_000227784.1 NZ_LR778141:c4958574-4958032 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT289109 WP_001297137.1 NZ_LR778141:c4958869-4958558 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References