Detailed information of TA system TA028910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2993267..2993946
• Replicon: chromosome
• Accession: NC_020245
• Organism: Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: K60_RS14120
• Protein ID: WP_003414059.1
• Coordinates: 2993267..2993683 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: K60_RS14125
• Protein ID: WP_003414061.1
• Coordinates: 2993680..2993946 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K60_RS14100	2989319..2990221	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
K60_RS14105	2990290..2991042	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
K60_RS14110	2991286..2991561	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
K60_RS14115	2991558..2993180	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
K60_RS14120	2993267..2993683	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
K60_RS14125	2993680..2993946	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K60_RS14130	2993972..2994367	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
K60_RS14135	2994364..2994633	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
K60_RS14140	2994643..2995737	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
K60_RS14145	2995734..2996153	-	420	Protein_2757	winged helix-turn-helix domain-containing protein	-
K60_RS14150	2996227..2996706	-	480	WP_003414073.1	dihydrofolate reductase	-
K60_RS14155	2996777..2997577	-	801	WP_003911953.1	thymidylate synthase	-
K60_RS14160	2997733..2998470	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T28910 WP_003414059.1 NC_020245:c2993683-2993267 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T28910 NC_020245:c2993683-2993267 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT28910 WP_003414061.1 NC_020245:c2993946-2993680 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT28910 NC_020245:c2993946-2993680 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64