Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4543381..4543878 Replicon chromosome
Accession NZ_LR778140
Organism Escherichia coli isolate SC406

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H1C94_RS21865 Protein ID WP_001173343.1
Coordinates 4543381..4543554 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H1C94_RS21870 Protein ID WP_000398619.1
Coordinates 4543582..4543878 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C94_RS21855 4540051..4541508 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H1C94_RS21860 4541745..4543262 + 1518 WP_001295074.1 lysine--tRNA ligase -
H1C94_RS21865 4543381..4543554 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H1C94_RS21870 4543582..4543878 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H1C94_RS21875 4544105..4544377 - 273 WP_000405647.1 N-acetyltransferase -
H1C94_RS21880 4544389..4544619 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
H1C94_RS21885 4544800..4546431 + 1632 WP_001216461.1 sensor histidine kinase -
H1C94_RS21890 4546428..4547147 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T289081 WP_001173343.1 NZ_LR778140:c4543554-4543381 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT289081 WP_000398619.1 NZ_LR778140:c4543878-4543582 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References