Detailed information of TA system TA289080

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4529651..4530486
• Replicon: chromosome
• Accession: NZ_LR778140
• Organism: Escherichia coli isolate SC406

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: H1C94_RS21815
• Protein ID: WP_061356574.1
• Coordinates: 4530109..4530486 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: H1C94_RS21810
• Protein ID: WP_061356575.1
• Coordinates: 4529651..4530019 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1C94_RS21770	4524930..4525610	+	681	WP_001282912.1	WYL domain-containing protein	-
H1C94_RS21775	4525761..4526438	+	678	WP_001097567.1	hypothetical protein	-
H1C94_RS21780	4526444..4526677	+	234	WP_001278282.1	DUF905 family protein	-
H1C94_RS21785	4526767..4527585	+	819	WP_115425218.1	DUF945 domain-containing protein	-
H1C94_RS21790	4527677..4528162	+	486	WP_000213706.1	antirestriction protein	-
H1C94_RS21795	4528177..4528653	+	477	WP_143370560.1	RadC family protein	-
H1C94_RS21800	4528716..4528937	+	222	WP_061356703.1	DUF987 domain-containing protein	-
H1C94_RS21805	4528956..4529601	+	646	Protein_4266	antitoxin of toxin-antitoxin stability system	-
H1C94_RS21810	4529651..4530019	+	369	WP_061356575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H1C94_RS21815	4530109..4530486	+	378	WP_061356574.1	type IV toxin-antitoxin system toxin CbtA	Toxin
H1C94_RS21820	4530483..4530971	+	489	WP_097743434.1	hypothetical protein	-
H1C94_RS21825	4530991..4531188	+	198	WP_097743433.1	DUF957 domain-containing protein	-
H1C94_RS21830	4531273..4532118	+	846	WP_097743432.1	DUF4942 domain-containing protein	-
H1C94_RS21835	4532189..4533724	+	1536	WP_053289162.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13988.94 Da
• Isoelectric Point: 7.8847

>T289080 WP_061356574.1 NZ_LR778140:4530109-4530486 [Escherichia coli]
MNTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITSGKHPEAKR

Nucleotide

• Length: 378 bp

>T289080 NZ_LR778140:4530109-4530486 [Escherichia coli]
ATGAATACATTACCTGTATTACCCGGACAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGTCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTCGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13602.30 Da
• Isoelectric Point: 5.9582

>AT289080 WP_061356575.1 NZ_LR778140:4529651-4530019 [Escherichia coli]
VSDTLSGTTHPDDNNERPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT289080 NZ_LR778140:4529651-4530019 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGACGACAACAACGAGCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA