Detailed information of TA system TA289050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4705448..4706785
• Replicon: chromosome
• Accession: NZ_LR745045
• Organism: Klebsiella pneumoniae isolate Kpn2166

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: GWJ69_RS23235
• Protein ID: WP_032424556.1
• Coordinates: 4705850..4706785 (+)
• Length: 312 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: GWJ69_RS23230
• Protein ID: WP_023302769.1
• Coordinates: 4705448..4705843 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWJ69_RS23200	4700692..4700982	+	291	WP_022615704.1	hypothetical protein	-
GWJ69_RS23205	4701047..4701952	+	906	WP_022615703.1	DUF1738 domain-containing protein	-
GWJ69_RS23210	4702145..4702516	+	372	WP_022615702.1	hypothetical protein	-
GWJ69_RS23215	4702690..4704285	+	1596	WP_023302771.1	TraI domain-containing protein	-
GWJ69_RS23220	4704457..4704921	+	465	WP_022615700.1	GNAT family N-acetyltransferase	-
GWJ69_RS23225	4704927..4705193	-	267	WP_023302770.1	hypothetical protein	-
GWJ69_RS23230	4705448..4705843	+	396	WP_023302769.1	hypothetical protein	Antitoxin
GWJ69_RS23235	4705850..4706785	+	936	WP_032424556.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GWJ69_RS23240	4706793..4707482	-	690	WP_023302767.1	hypothetical protein	-
GWJ69_RS23245	4707657..4708451	-	795	WP_023302766.1	RES domain-containing protein	-
GWJ69_RS23250	4708870..4710549	-	1680	WP_004212561.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-298)

Antitoxin

• Protein of unknown function DUF6088 (7-109)

Sequences

Toxin

Protein

• Length: 312 a.a.
• Molecular weight: 35389.66 Da
• Isoelectric Point: 5.6165

>T289050 WP_032424556.1 NZ_LR745045:4705850-4706785 [Klebsiella pneumoniae]
MLFSDVTDALGIESGAIVEKDHYIVELLHLLKPLTFDTHQLLFAGGTALAKSGIVLNRMSEDVDIKLVPTDTFLSLSRTQ
RKGIRKEIVQTISHAITATDMFSFDDAHPKVTRDEYRYNDIPVRYPQRVAQAPCLRPFIQLELMEANLLEAPEFRDIHTL
VTTLTGKAPVISAFPCATIISTQAEKLVAMLRRTAAVARNIERQDDASLVRHIYDTYCIVQAGNVDMCLLTDFFKQTIEQ
DIQRYGNQYPQFCVSPNDELKMGLDELEHNPLYKTRYQQFVAPMVYGARKVSWDEAYASFRQMALIILDTY

Nucleotide

• Length: 936 bp

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14800.08 Da
• Isoelectric Point: 11.1078

>AT289050 WP_023302769.1 NZ_LR745045:4705448-4705843 [Klebsiella pneumoniae]
MTIKTRIQSRLKRSKRYVFTRDDFKDIAGYDQIGRVLRDLLKEGQLLKVGYGVYTKARRNSITGKVMPAAPRGSSAVIVE
TLERLKVRYRYEGATQTYNSGESTQIPVSPEIKTSPRFKRALSVGNSKLNG

Nucleotide

• Length: 396 bp