Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1828265..1828855 Replicon chromosome
Accession NZ_LR745045
Organism Klebsiella pneumoniae isolate Kpn2166

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag GWJ69_RS09030 Protein ID WP_022615588.1
Coordinates 1828523..1828855 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag GWJ69_RS09025 Protein ID WP_000288812.1
Coordinates 1828265..1828522 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWJ69_RS09005 1824600..1826348 + 1749 WP_032457402.1 hypothetical protein -
GWJ69_RS09010 1826427..1826983 + 557 Protein_1736 hypothetical protein -
GWJ69_RS09015 1827266..1827727 + 462 WP_004213450.1 hypothetical protein -
GWJ69_RS09020 1827724..1827930 + 207 WP_022615589.1 helix-turn-helix domain-containing protein -
GWJ69_RS09025 1828265..1828522 + 258 WP_000288812.1 antitoxin Antitoxin
GWJ69_RS09030 1828523..1828855 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GWJ69_RS09045 1829177..1830613 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
GWJ69_RS09055 1830979..1832433 - 1455 WP_004148975.1 AMP nucleosidase -
GWJ69_RS09060 1832563..1832808 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T289042 WP_022615588.1 NZ_LR745045:1828523-1828855 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT289042 WP_000288812.1 NZ_LR745045:1828265-1828522 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References