Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2741527..2742179 Replicon chromosome
Accession NC_020245
Organism Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag K60_RS12820 Protein ID WP_003412752.1
Coordinates 2741754..2742179 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag K60_RS12815 Protein ID WP_003412749.1
Coordinates 2741527..2741748 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K60_RS12800 2739767..2739973 - 207 WP_003899347.1 hypothetical protein -
K60_RS12805 2740109..2740732 + 624 WP_003900858.1 TIGR00725 family protein -
K60_RS12810 2740722..2741474 + 753 WP_003901416.1 hypothetical protein -
K60_RS12815 2741527..2741748 + 222 WP_003412749.1 antitoxin Antitoxin
K60_RS12820 2741754..2742179 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
K60_RS12825 2742202..2743383 - 1182 WP_010950729.1 2-oxo acid dehydrogenase subunit E2 -
K60_RS12830 2743380..2744426 - 1047 WP_010950730.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
K60_RS12835 2744437..2745540 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
K60_RS12840 2745799..2746620 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
K60_RS12845 2746617..2747174 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T28904 WP_003412752.1 NC_020245:2741754-2742179 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T28904 NC_020245:2741754-2742179 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT28904 WP_003412749.1 NC_020245:2741527-2741748 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT28904 NC_020245:2741527-2741748 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References