Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4573267..4574077 Replicon chromosome
Accession NZ_LR745042
Organism Klebsiella pneumoniae isolate Klebsiella pneumoniae kpn154

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag GW691_RS22505 Protein ID WP_004178461.1
Coordinates 4573267..4573800 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GW691_RS22510 Protein ID WP_002887278.1
Coordinates 4573811..4574077 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW691_RS22500 4572098..4573219 + 1122 WP_004214138.1 cupin domain-containing protein -
GW691_RS22505 4573267..4573800 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
GW691_RS22510 4573811..4574077 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
GW691_RS22515 4574180..4575613 - 1434 WP_004214141.1 Cu(+)/Ag(+) sensor histidine kinase -
GW691_RS22520 4575603..4576286 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
GW691_RS22525 4576458..4577843 + 1386 WP_004214145.1 efflux transporter outer membrane subunit -
GW691_RS22530 4577861..4578205 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T289032 WP_004178461.1 NZ_LR745042:c4573800-4573267 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT289032 WP_002887278.1 NZ_LR745042:c4574077-4573811 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References