28898

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2182605..2183191 Replicon chromosome
Accession NC_020245
Organism Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag K60_RS10160 Protein ID WP_003410010.1
Coordinates 2182605..2182949 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag K60_RS10165 Protein ID WP_003410014.1
Coordinates 2182943..2183191 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K60_RS10130 2179506..2179811 + 306 Protein_1978 ABC transporter permease -
K60_RS10135 2179995..2180519 + 525 WP_011799234.1 methyltransferase -
K60_RS10140 2181039..2181599 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
K60_RS10145 2181596..2181937 - 342 WP_003410003.1 DUF2384 domain-containing protein -
K60_RS10150 2182023..2182280 + 258 WP_003410006.1 hypothetical protein -
K60_RS10155 2182181..2182516 - 336 WP_003410009.1 dehydrogenase -
K60_RS10160 2182605..2182949 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
K60_RS10165 2182943..2183191 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
K60_RS10170 2183291..2185606 - 2316 WP_003899120.1 cation transporter ATPase CptG -
K60_RS10175 2185603..2185875 - 273 WP_003410017.1 DUF1490 family protein -
K60_RS10180 2185928..2186284 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
K60_RS10185 2186441..2187208 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T28898 WP_003410010.1 NC_020245:c2182949-2182605 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T28898 NC_020245:c2182949-2182605 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT28898 WP_003410014.1 NC_020245:c2183191-2182943 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT28898 NC_020245:c2183191-2182943 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAW9

References