Detailed information of TA system TA028894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2166449..2167152
• Replicon: chromosome
• Accession: NC_020245
• Organism: Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: K60_RS10005
• Protein ID: WP_003409778.1
• Coordinates: 2166449..2166778 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: K60_RS21080
• Protein ID: WP_003409780.1
• Coordinates: 2166775..2167152 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K60_RS09985	2162832..2163902	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
K60_RS09990	2163899..2164414	+	516	WP_003409718.1	flavin reductase family protein	-
K60_RS09995	2164411..2165472	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
K60_RS10000	2165469..2166239	+	771	WP_003409775.1	SDR family oxidoreductase	-
K60_RS10005	2166449..2166778	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K60_RS21080	2166775..2167152	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
K60_RS10015	2167149..2167739	-	591	WP_003910477.1	SEC-C domain-containing protein	-
K60_RS10020	2167794..2169158	+	1365	WP_011799233.1	HNH endonuclease	-
K60_RS10025	2169313..2169765	-	453	WP_010950636.1	lipoprotein	-
K60_RS10030	2169829..2170230	+	402	WP_003409869.1	hypothetical protein	-
K60_RS10035	2170223..2170405	-	183	WP_003409870.1	hypothetical protein	-
K60_RS10040	2170519..2170869	-	351	WP_003409871.1	hypothetical protein	-
K60_RS10045	2170880..2171782	-	903	WP_003409874.1	hypothetical protein	-
K60_RS10050	2171803..2171994	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T28894 WP_003409778.1 NC_020245:c2166778-2166449 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T28894 NC_020245:c2166778-2166449 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT28894 WP_003409780.1 NC_020245:c2167152-2166775 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT28894 NC_020245:c2167152-2166775 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8