Detailed information of TA system TA028891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1749492..1750105
• Replicon: chromosome
• Accession: NC_020245
• Organism: Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: K60_RS08180
• Protein ID: WP_003407786.1
• Coordinates: 1749701..1750105 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: K60_RS08175
• Protein ID: WP_009935474.1
• Coordinates: 1749492..1749695 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K60_RS08160	1744756..1747659	+	2904	WP_003407779.1	MMPL family transporter	-
K60_RS08165	1747669..1748115	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
K60_RS08170	1748150..1749439	+	1290	WP_003407781.1	threonine ammonia-lyase	-
K60_RS08175	1749492..1749695	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K60_RS08180	1749701..1750105	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
K60_RS08185	1750122..1751863	-	1742	Protein_1592	malto-oligosyltrehalose trehalohydrolase	-
K60_RS08190	1751856..1754153	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T28891 WP_003407786.1 NC_020245:1749701-1750105 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T28891 NC_020245:1749701-1750105 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT28891 WP_009935474.1 NC_020245:1749492-1749695 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT28891 NC_020245:1749492-1749695 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46