Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1681573..1682189 Replicon chromosome
Accession NC_020245
Organism Mycobacterium bovis BCG str. Korea 1168P

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag K60_RS07905 Protein ID WP_003407593.1
Coordinates 1681872..1682189 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7U4BU54
Locus tag K60_RS07900 Protein ID WP_011799188.1
Coordinates 1681573..1681875 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K60_RS07890 1677459..1679306 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
K60_RS07895 1679307..1681559 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
K60_RS07900 1681573..1681875 + 303 WP_011799188.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
K60_RS07905 1681872..1682189 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
K60_RS07910 1682186..1683190 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
K60_RS07915 1683243..1684532 + 1290 WP_003407599.1 serine hydrolase -
K60_RS07920 1684605..1685378 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
K60_RS07925 1685436..1685627 - 192 WP_003407609.1 dodecin family protein -
K60_RS22285 1685658..1685882 - 225 WP_160522526.1 NUDIX domain-containing protein -
K60_RS07935 1686152..1687180 + 1029 WP_011799189.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T28890 WP_003407593.1 NC_020245:1681872-1682189 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T28890 NC_020245:1681872-1682189 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11199.50 Da        Isoelectric Point: 9.5564

>AT28890 WP_011799188.1 NC_020245:1681573-1681875 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
VPFLVALSGIILGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT28890 NC_020245:1681573-1681875 [Mycobacterium tuberculosis variant bovis BCG str. Korea 1168P]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTTGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BU54

References