Detailed information of TA system TA288780

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 574878..575539
• Replicon: chromosome
• Accession: NZ_LR699014
• Organism: Citrobacter werkmanii isolate MGYG-HGUT-02535

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: S3IMJ6
• Locus tag: FYC27_RS02860
• Protein ID: WP_000698542.1
• Coordinates: 575216..575539 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: S3J179
• Locus tag: FYC27_RS02855
• Protein ID: WP_000065326.1
• Coordinates: 574878..575195 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYC27_RS02810	570018..570842	+	825	WP_000197385.1	DUF945 domain-containing protein	-
FYC27_RS02815	571051..571761	+	711	WP_024198336.1	hypothetical protein	-
FYC27_RS02820	571787..572323	+	537	WP_000219797.1	DUF4339 domain-containing protein	-
FYC27_RS02825	572365..572802	+	438	WP_024198335.1	hypothetical protein	-
FYC27_RS02830	572869..573279	+	411	WP_000912997.1	hypothetical protein	-
FYC27_RS02835	573357..573593	+	237	WP_000004273.1	DUF905 domain-containing protein	-
FYC27_RS02840	573679..574137	+	459	WP_016538084.1	antirestriction protein	-
FYC27_RS02845	574153..574629	+	477	WP_000811694.1	RadC family protein	-
FYC27_RS02850	574638..574859	+	222	WP_000691995.1	DUF987 domain-containing protein	-
FYC27_RS02855	574878..575195	+	318	WP_000065326.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FYC27_RS02860	575216..575539	+	324	WP_000698542.1	TA system toxin CbtA family protein	Toxin
FYC27_RS02865	576049..579591	-	3543	WP_096755809.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	547266..575539	28273

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12350.38 Da
• Isoelectric Point: 8.9789

>T288780 WP_000698542.1 NZ_LR699014:575216-575539 [Citrobacter werkmanii]
MKILPATISRAAKPCLPPVAVWQLLLTRLLEKHYGLTLNDTPFSDETVIKEHFDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTNIDIMRARRDLGLLNRN

Nucleotide

• Length: 324 bp

>T288780 NZ_LR699014:575216-575539 [Citrobacter werkmanii]
ATGAAAATTCTACCGGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGCCACCCGTTGCAGTCTGGCAATTGCTTCTTAC
ACGGCTACTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCAGTGATGAAACCGTTATAAAGGAACATTTCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATATCTCACGAATATTGATATCATGAGGGCCCGCCGCGATCTGGGTTTATTAAATCGTAA
TTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11830.50 Da
• Isoelectric Point: 6.9367

>AT288780 WP_000065326.1 NZ_LR699014:574878-575195 [Citrobacter werkmanii]
MSNIIWGLQRDITPRLGTRLVQEGNQLHYLADRACITGKFSDAECLKLDVAFPHFISRMESMLSTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYVAVYSTQR

Nucleotide

• Length: 318 bp

>AT288780 NZ_LR699014:574878-575195 [Citrobacter werkmanii]
ATGAGCAACATCATTTGGGGCCTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTACCTGGCTGACCGGGCCTGTATCACAGGTAAGTTCAGTGATGCCGAATGTCTTAAGCTGGATGTGGCATTTCCGC
ATTTTATCAGCCGGATGGAATCCATGCTGAGCACGGGTGAAATGAATCCTCGCCACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGTGGCTATGTATACGTCGCTGTTTATTCCACTCAGCGCTAA

Structures

Toxin

• AlphaFold DB: S3IMJ6

Antitoxin

• AlphaFold DB: S3J179