Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 40939..41582 Replicon plasmid pEcMAD1
Accession NZ_LR595692
Organism Escherichia coli isolate EcMAD1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag ECMAD3_RS23310 Protein ID WP_001044768.1
Coordinates 40939..41355 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag ECMAD3_RS23315 Protein ID WP_001261287.1
Coordinates 41352..41582 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMAD3_RS23295 36243..37175 - 933 WP_000991832.1 hypothetical protein -
ECMAD3_RS23300 37179..38174 - 996 WP_000246636.1 hypothetical protein -
ECMAD3_RS23305 38639..40777 + 2139 WP_000350635.1 AAA family ATPase -
ECMAD3_RS23310 40939..41355 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
ECMAD3_RS23315 41352..41582 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECMAD3_RS23325 41889..45008 + 3120 WP_023909028.1 hypothetical protein -
ECMAD3_RS23330 45271..46404 + 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / aac(3)-IId / blaTEM-1B / aadA5 / qacE / sul1 / tet(B) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / sul2 / aph(3'')-Ib / aph(6)-Id - 1..98473 98473
- flank IS/Tn - - 35603..36106 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T288617 WP_001044768.1 NZ_LR595692:c41355-40939 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT288617 WP_001261287.1 NZ_LR595692:c41582-41352 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References