Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3060199..3060853 Replicon chromosome
Accession NZ_LR595691
Organism Escherichia coli isolate EcMAD1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECMAD3_RS14765 Protein ID WP_000244777.1
Coordinates 3060199..3060606 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECMAD3_RS14770 Protein ID WP_000354046.1
Coordinates 3060587..3060853 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMAD3_RS14745 3056156..3057889 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
ECMAD3_RS14750 3057895..3058605 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECMAD3_RS14755 3058630..3059526 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECMAD3_RS14760 3059638..3060159 + 522 WP_001055874.1 flavodoxin FldB -
ECMAD3_RS14765 3060199..3060606 - 408 WP_000244777.1 toxin CptA Toxin
ECMAD3_RS14770 3060587..3060853 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECMAD3_RS14775 3061096..3062076 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECMAD3_RS14780 3062153..3062812 - 660 WP_000250274.1 hemolysin III family protein -
ECMAD3_RS14785 3062976..3063287 - 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
ECMAD3_RS14790 3063332..3064765 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
ECMAD3_RS14795 3064822..3065565 - 744 Protein_2851 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T288610 WP_000244777.1 NZ_LR595691:c3060606-3060199 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT288610 WP_000354046.1 NZ_LR595691:c3060853-3060587 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References