Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 511999..512635 Replicon chromosome
Accession NC_020244
Organism Bacillus subtilis XF-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag C663_RS02535 Protein ID WP_003156187.1
Coordinates 512285..512635 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag C663_RS02530 Protein ID WP_003225183.1
Coordinates 511999..512280 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C663_RS02510 508406..508957 - 552 Protein_461 rhomboid family intramembrane serine protease -
C663_RS02515 509052..509417 + 366 WP_015252768.1 holo-ACP synthase -
C663_RS02520 509583..510599 + 1017 WP_080030712.1 outer membrane lipoprotein carrier protein LolA -
C663_RS02525 510714..511883 + 1170 WP_015382833.1 alanine racemase -
C663_RS02530 511999..512280 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
C663_RS02535 512285..512635 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C663_RS02540 512751..513575 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
C663_RS02545 513580..513945 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
C663_RS02550 513949..514350 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
C663_RS02555 514362..515369 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
C663_RS02560 515431..515760 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
C663_RS02565 515757..516239 + 483 WP_003234299.1 anti-sigma B factor RsbW -
C663_RS02570 516205..516993 + 789 WP_015482833.1 RNA polymerase sigma factor SigB -
C663_RS02575 516993..517592 + 600 WP_015382836.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T28861 WP_003156187.1 NC_020244:512285-512635 [Bacillus subtilis XF-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T28861 NC_020244:512285-512635 [Bacillus subtilis XF-1]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT28861 WP_003225183.1 NC_020244:511999-512280 [Bacillus subtilis XF-1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT28861 NC_020244:511999-512280 [Bacillus subtilis XF-1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References