Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2619249..2619874 Replicon chromosome
Accession NZ_LR595691
Organism Escherichia coli isolate EcMAD1

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag ECMAD3_RS12645 Protein ID WP_000911330.1
Coordinates 2619249..2619647 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag ECMAD3_RS12650 Protein ID WP_000450524.1
Coordinates 2619647..2619874 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMAD3_RS12625 2614966..2615712 + 747 WP_053906593.1 IS21-like element ISEc12 family helper ATPase IstB -
ECMAD3_RS12630 2615794..2616135 - 342 Protein_2437 esterase -
ECMAD3_RS12635 2616209..2618224 - 2016 WP_000829323.1 tRNA cytosine(34) acetyltransferase TmcA -
ECMAD3_RS12640 2618239..2619102 - 864 WP_001267507.1 neutral zinc metallopeptidase -
ECMAD3_RS12645 2619249..2619647 - 399 WP_000911330.1 tRNA(fMet)-specific endonuclease VapC Toxin
ECMAD3_RS12650 2619647..2619874 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
ECMAD3_RS12655 2620028..2620741 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
ECMAD3_RS12660 2620954..2621988 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
ECMAD3_RS12665 2622005..2622883 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
ECMAD3_RS12670 2623029..2623601 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
ECMAD3_RS12675 2623601..2624071 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T288607 WP_000911330.1 NZ_LR595691:c2619647-2619249 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT288607 WP_000450524.1 NZ_LR595691:c2619874-2619647 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References