Detailed information of TA system TA288600

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1157860..1158704
• Replicon: chromosome
• Accession: NZ_LR595691
• Organism: Escherichia coli isolate EcMAD1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: ECMAD3_RS05540
• Protein ID: WP_000854686.1
• Coordinates: 1158321..1158704 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: ECMAD3_RS05535
• Protein ID: WP_001285602.1
• Coordinates: 1157860..1158240 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECMAD3_RS05495	1154108..1154563	+	456	WP_000581506.1	hypothetical protein	-
ECMAD3_RS05500	1154664..1154872	+	209	Protein_1050	DUF905 family protein	-
ECMAD3_RS05510	1154973..1155794	+	822	WP_001761104.1	DUF945 domain-containing protein	-
ECMAD3_RS05515	1155886..1156371	+	486	WP_000214307.1	antirestriction protein	-
ECMAD3_RS05520	1156387..1156863	+	477	WP_001186726.1	RadC family protein	-
ECMAD3_RS05525	1156926..1157147	+	222	WP_000692298.1	DUF987 domain-containing protein	-
ECMAD3_RS05530	1157166..1157849	+	684	WP_000086768.1	hypothetical protein	-
ECMAD3_RS05535	1157860..1158240	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECMAD3_RS05540	1158321..1158704	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
ECMAD3_RS05545	1158701..1159189	+	489	WP_032180032.1	hypothetical protein	-
ECMAD3_RS05550	1159206..1159403	+	198	WP_000839253.1	DUF957 domain-containing protein	-
ECMAD3_RS05555	1159488..1160329	+	842	Protein_1060	DUF4942 domain-containing protein	-
ECMAD3_RS05565	1160800..1161738	+	939	WP_000351311.1	glyoxylate/hydroxypyruvate reductase GhrA	-
ECMAD3_RS05570	1161793..1162530	+	738	WP_000283664.1	zinc-binding phosphatase	-
ECMAD3_RS05575	1162554..1163108	+	555	WP_001001902.1	molecular chaperone YcdY	-
ECMAD3_RS05580	1163210..1163701	+	492	WP_001297187.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T288600 WP_000854686.1 NZ_LR595691:1158321-1158704 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT288600 WP_001285602.1 NZ_LR595691:1157860-1158240 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp