288599

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 1137779..1139499 Replicon chromosome
Accession NZ_LR595691
Organism Escherichia coli isolate EcMAD1

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag ECMAD3_RS05440 Protein ID WP_000312833.1
Coordinates 1138843..1139499 (-) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag ECMAD3_RS05435 Protein ID WP_000634203.1
Coordinates 1137779..1138846 (-) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMAD3_RS05415 1132990..1133604 - 615 Protein_1035 IS3-like element IS600 family transposase -
ECMAD3_RS05425 1133949..1134665 - 717 WP_001122107.1 M48 family metallopeptidase -
ECMAD3_RS05430 1134665..1137760 - 3096 WP_000108736.1 HsdR family type I site-specific deoxyribonuclease -
ECMAD3_RS05435 1137779..1138846 - 1068 WP_000634203.1 macro domain-containing protein Antitoxin
ECMAD3_RS05440 1138843..1139499 - 657 WP_000312833.1 DUF4433 domain-containing protein Toxin
ECMAD3_RS05445 1139512..1140855 - 1344 WP_032180130.1 restriction endonuclease subunit S -
ECMAD3_RS05450 1140867..1143305 - 2439 WP_032180025.1 type I restriction-modification system subunit M -
ECMAD3_RS05455 1143345..1143584 - 240 WP_024224214.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage blaCMY-2 - 1116658..1146937 30279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T288599 WP_000312833.1 NZ_LR595691:c1139499-1138843 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT288599 WP_000634203.1 NZ_LR595691:c1138846-1137779 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References